AOAC

Tuberculocidal Activity of Disinfectants (AOAC 965.12)

The AOAC Tuberculocidal Activity of Disinfectants method is specified by US EPA as a method which can be used to substantiate tuberculocidal efficacy claims for disinfectants. It is a modified version of the AOAC Use-Dilution test method and particularly appropriate for dilutable disinfectants. Summary of the AOAC Tuberculocidal Activity of...

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Pre-Saturated Towelette Disinfection Test

The EPA pre-saturated towelette disinfection test method is used to substantiate efficacy claims for disinfectant towelettes.     This method is a modification of the AOAC Germicidal Spray Products Test for Disinfectants. Instead of contaminated test surfaces being sprayed with disinfectant as in the germicidal spray products test, they are wiped with...

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Fungicidal Activity of Disinfectants (AOAC Method 955.17)

The AOAC Fungicidal Activity of Disinfectants Test method examines the fungicidal capabilities of water-soluble fungicides intended for use in disinfection of inanimate objects. The AOAC Fungicidal Activity of Disinfectants Test is specified by the US EPA as a method which can be used to substantiate fungicidal efficacy claims. Below, you...

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AOAC Germicidal and Detergent Sanitizing Action of Disinfectants (AOAC 960.09)

The AOAC Germicidal and Detergent Sanitizing Action of Disinfectants Test Method is specified by US EPA as a method which can be used to substantiate efficacy claims for certain food-contact surface sanitizers. It is particularly appropriate for dilutable sanitizers that do not utilize halogens (chlorine, bromine). Test Method Summary Cultures...

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AOAC Germicidal Spray Products as Disinfectants Test

Procedure At A Glance For this method, sterile glass cover slips (carriers) are transferred into sterile petri plates then inoculated with 10 μl of the test bacteria. For products that only have a sanitizing/disinfecting claim, meaning a cleaning step to remove organic soil that precedes the sanitizing/disinfecting of the contaminated...

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AOAC Germicidal Spray Products Test (AOAC 961.02)

Originally developed in 1961, the AOAC Germicidal Spray Test is a standard method for evaluating the efficacy of liquid disinfectants on hard, non-porous surfaces. It is one of the EPA-specified test methods for antimicrobial pesticide registration. This method is particularly appropriate for disinfectants which do not require dilution and...

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AOAC Germicidal Spray Test Modified for Fungi

Below, you will find a summary of the AOAC Germicidal Spray Products Test method, modified for use with fungi. The method is sometimes modified for use with fungi to support supplemental efficacy (fungicidal) label claims for pesticides registered with the United States Environmental Protection Agency. Trichophyton mentagtrophytes is the preferred test...

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AOAC Use Dilution Test (AOAC 955.14; 955.15; 964.02)

The AOAC Use Dilution method is a method of testing the efficacy of disinfectants, originally developed in 1955. Throughout its numerous revisions, it has become the standard for evaluating liquid and dilutable liquid disinfectants for hard surfaces. In particular, this method is specified by the U.S. EPA as the...

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AOAC Use-Dilution Test

AOAC Use Dilution Test: Procedure at a GlanceFor this study, bactericidal activity is measured qualitatively against various microorganisms.  Sterile test surfaces called penicylinders are inoculated with culture, dried, and then exposed to the test disinfectant at the label claim contact temperature and time.  The carriers are then transferred to...

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Chlorine Equivalency Method for Sanitizers (AOAC 955.16)

Officially called the Chlorine (available) in Disinfectants Germicidal Equivalent Concentration, AOAC method 955.16 is typically used to test the efficacy of halogens such as chlorine, bromine, iodine. It is a rather unique method among disinfectant and sanitizer test methods, designed to identify the amount of a test product that is...

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EPA Disinfectant Towelette Test

EPA Towelette Test: Procedure at a glanceThe typical bacteria used in this test, S. aureus, P. aeruginosa, and S. enterica are representative Gram-positive and Gram-negative pathogenic bacteria. The microorganisms are inoculated and dried onto the surface of glass microscope slides, the carrier. The slides are then wiped with pre-saturated disinfectant...

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AOAC 955.17 Fungicidal Activity of Disinfectants

This test is used to assess the fungicidal activity of a disinfectant. In this test, a typical fungus strain of Trichophyton mentagrophytes is cultured and resuspended in order to attain spore producing mycelium; however, the method can be modified to meet the needs of the study sponsor's requirements. The...

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AOAC 966.04 Sporicidal Activity Test

AOAC 966.04 Sporicidal Activity Test: Procedure at a GlanceEssential for the registration and verification of sporicidal claims with the EPA as a countertop disinfectant and the FDA as a high-level disinfectant, the AOAC 966.04 Sporicidal Activity Test uses either Bacillus subtilis or Clostridium sporogenes as the model organism for...

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