ASTM

ASTM E2180 Test for Hydrophobic Antimicrobial Surfaces

The ASTM E2180 method is designed to quantitatively test the antimicrobial effectiveness of incorporated antimicrobial agent(s) in polymeric or hydrophobic materials. Bacterial inocula (which are aqueous solutions) may bead on hydrophobic test surfaces, creating a domed or nearly spherical shaped droplet, thereby reducing bacterial contact with the treated surface. This...

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ASTM E2197 – Quantitative Disk Carrier Test

The ASTM E2197 method is designed to test bactericidal, fungicidal, sporicidal, and virucidal capabilities of liquid germicides on non-porous hard surfaces using a 1 cm diameter brushed stainless steel surface. This method has been accepted by the U.S. EPA to substantiate efficacy claims for disinfectants against endospores of Clostridium difficile. The method...

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ASTM D3273 Fungal Resistance Test for Coated Surfaces

The ASTM D3273 method is meant to evaluate fungal defacement of coated interior surfaces in an accelerated manner. The ASTM D3273 test specifically aims to establish the relative resistance of paint films to the propagation of mold and mildew under optimum fungal growth conditions. Incubation occurs in a highly...

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ASTM E2197 Disk-Carrier Test for C. difficile

ASTM E2197 Test: Procedure at a GlanceIn this study, efficacy of a test disinfectant is measured qualitatively against various vegetative bacteria, viruses, fungi, mycobacteria, or bacterial spores.  For EPA registration the disinfectant is tested at the lower certified limits (or minimum recommended concentration MRC), a dilution of the test...

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ASTM D3274 Fungal Defacement Rating Method

The ASTM D3274 Method details a standardized rating system designed to evaluate the degree of fungal resistance of surface coatings (e.g. paint films). This method is used for tests such as the ASTM D3273. Ratings are based on the percentage of surface fungal coverage and qualitative descriptions. While this...

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ASTM E2315 – Liquid Suspension Time-Kill Test

The liquid suspension time-kill test is excellent for disinfectant product developers because it is a fast, relatively inexpensive, and reproducible way to measure the biocidal potential of a liquid antimicrobial formulation. It consists of directly inoculating a liquid test substance with a high concentration of test microorganisms and then...

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ASTM E1052 – Suspension Time-Kill Test for Virus

Summary of the ASTM E1052 Test ASTM E1052: Standard Practice to Assess the Activity of Microbicides Against Virucides in Suspension The frozen stock test virus is thawed and diluted to a titer of approximately 6-log10 infectious units per 0.1 ml. If requested by the Study Sponsor, the test virus is...

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ASTM E2315 Time-Kill Test

ASTM E2315: Procedure at a GlanceThe Liquid Suspension Time-Kill begins with the direct inoculation of a volume of the disinfectant in question with a specified representative organism. After a specified contact time the system is neutralized and the presence of microorganisms is monitored and quantified following a period of...

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ASTM E1053 – Surface Time Kill Test for Viruses

Summary of the ASTM E1053 Method ASTM E1053: Virucidal Efficacy of Hard Surface Disinfectants - Column Neutralization Preparation of Virus and Inoculation of Carriers: 100 x 15 mm Petri dishes are used for liquid and spray products, and 150 x 20 mm glass Petri dishes are used for towelettes. Just before...

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ASTM G21 Fungal Defacement Test

The ASTM G 21 method, titled "Standard Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi" is designed to test the resistance of synthetic polymeric materials (molded materials, surface treated articles or similar objects) to fungal attack. It is a popular method, used by industry to evaluate the resistance...

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ASTM E1054 Neutralization Evaluation Test

The method is designed to test neutralizing agents for the purpose of inactivating, or quenching, active antimicrobial agents to ensure no inhibitory effects on microorganisms targeted for recovery. The method offers a wide array of neutralization options for test substances. Detailed within the method are the guidelines for neutralization...

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ASTM G29 Test for Resistance to Algae

The ASTM G29 test method evaluates an antimicrobial surface's ability to resist algal attack. Such testing is important because algae are ubiquitously found in and around bodies of water. Many of these aqueous environments are associated with human activities, such as swimming pools. Under the right conditions, algae can...

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ASTM E1153 – EPA Non-Food Contact Sanitizer Test

The ASTM E1153 is a test method recognized by the EPA for the claim substantiation of sanitizers for use on non-food contact surfaces. For this reason, it is often referred to as the "non-food contact sanitizer" test. This test is performed by drying bacteria onto glass carriers, treating them with...

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ASTM E1153 Non-Food Contact Sanitizer Test

ASTM E1153: Procedure At a GlanceIn this study, carriers/surfaces will be inoculated with 10 µl of Staphylococcus aureus and Klebsiella pneumoniae (or Enterobacter aerogenes substituted) and dried for 35 minutes at 35 ± 2ºC.  The surfaces will be transferred into sterile jars, petri plates, or other appropriate sterile container...

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ASTM E1428 “Pink Stain Test”

Some materials may become discolored or stained when microorganisms grow on them in the environment. The ASTM E1428 standard is used to evaluate the ability of synthetic polymeric materials containing antimicrobial agents to resist such staining, using the indicator staining organism Streptoverticullium reticulum. The ASTM E1428 method is straightforward and...

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ASTM E2149

The ASTM E2149 method, titled "Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions" is a sensitive test, often used to measure the antimicrobial activity of non-leaching, irregularly shaped or hydrophobic surfaces. Put simply, it measures the antimicrobial activity of antimicrobial surfaces when they're shaken around in a...

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ASTM G22 Resistance of Plastics to Bacteria

The ASTM G 22 test method, titled "Standard Practice for Determining Resistance of Plastics to Bacteria" is designed to test the resistance of synthetic polymeric materials (molded materials, surface treated articles or similar objects) to bacterial attack.The main component of synthetic polymeric materials, resin, is usually resistant to bacteria,...

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