Disinfectant

EPA Residual Self-Disinfectant (RSD) Method

The residual self-disinfection (RSD) method follows EPA Protocol # 01-1A. This is the only method which can currently be used to substantiate long-term residual disinfectant claims with the EPA. It evaluates the residual disinfectant efficacy of antimicrobial products after application to inanimate, nonporous, and non-food contact hard surfaces. This test...

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Rinsability Test

In the usage of high-level disinfectants, the possibility of contact with these substances is something that needs to be ensured does not occur if the active ingredient could be harmful. To ensure that the level of active ingredient in/on the surface in question is at a level that would...

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Tuberculocidal Activity of Disinfectants (AOAC 965.12)

The AOAC Tuberculocidal Activity of Disinfectants method is specified by US EPA as a method which can be used to substantiate tuberculocidal efficacy claims for disinfectants. It is a modified version of the AOAC Use-Dilution test method and particularly appropriate for dilutable disinfectants. Summary of the AOAC Tuberculocidal Activity of...

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Virucidal Towelettes Effectiveness Test

Virucidal Efficacy Guidelines The U.S. EPA recommends the use of internationally-recognized standard test methods as the basis for pre-saturated towelette virucidal efficacy evaluations: AOAC Germicidal Spray Products as Disinfectants Test, Modified for Wipes Brief Summary of the Test The U.S. EPA recommends the use of internationally-recognized standard test methods as the basis for...

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Fungicidal Activity of Disinfectants (AOAC Method 955.17)

The AOAC Fungicidal Activity of Disinfectants Test method examines the fungicidal capabilities of water-soluble fungicides intended for use in disinfection of inanimate objects. The AOAC Fungicidal Activity of Disinfectants Test is specified by the US EPA as a method which can be used to substantiate fungicidal efficacy claims. Below, you...

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Hard Surface Time-Kill Test for Disinfectants

Summary of the Hard Surface Time-Kill Test     A microbial culture is prepared. For most bacteria, a 24 hour culture in nutrient broth works well. For most fungi, a spore preparation from a saline wash works well.   A volume of microbial culture (usually 0.010 ml to 0.020 ml) is placed onto the...

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Material Compatibilty Soak Test

In order to make claim that a material is compatible with a specific substance it is always good to have a firm understanding of just how much exposure a material can withstand without any detrimental interactions. At Microchem Laboratory, we can custom tailor a material compatibility soak test to...

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Mechanical Abrasion Test

Mechanical Abrasion Test: Procedure at a Glance In the standardized testing of surfaces with real-world applications it is important not to lose sight of the real-world conditions these surfaces will face. At Microchem Laboratory, the mechanical abrasion test seeks to recreate the cleaning conditions that materials will see on a...

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Pre-Saturated Towelette Disinfection Test

The EPA pre-saturated towelette disinfection test method is used to substantiate efficacy claims for disinfectant towelettes.     This method is a modification of the AOAC Germicidal Spray Products Test for Disinfectants. Instead of contaminated test surfaces being sprayed with disinfectant as in the germicidal spray products test, they are wiped with...

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AOAC Use-Dilution Test

AOAC Use Dilution Test: Procedure at a GlanceFor this study, bactericidal activity is measured qualitatively against various microorganisms.  Sterile test surfaces called penicylinders are inoculated with culture, dried, and then exposed to the test disinfectant at the label claim contact temperature and time.  The carriers are then transferred to...

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EPA Disinfectant Towelette Test

EPA Towelette Test: Procedure at a glanceThe typical bacteria used in this test, S. aureus, P. aeruginosa, and S. enterica are representative Gram-positive and Gram-negative pathogenic bacteria. The microorganisms are inoculated and dried onto the surface of glass microscope slides, the carrier. The slides are then wiped with pre-saturated disinfectant...

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ASTM E1052 – Suspension Time-Kill Test for Virus

Summary of the ASTM E1052 Test ASTM E1052: Standard Practice to Assess the Activity of Microbicides Against Virucides in Suspension The frozen stock test virus is thawed and diluted to a titer of approximately 6-log10 infectious units per 0.1 ml. If requested by the Study Sponsor, the test virus is...

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ASTM E1053 – Surface Time Kill Test for Viruses

Summary of the ASTM E1053 Method ASTM E1053: Virucidal Efficacy of Hard Surface Disinfectants - Column Neutralization Preparation of Virus and Inoculation of Carriers: 100 x 15 mm Petri dishes are used for liquid and spray products, and 150 x 20 mm glass Petri dishes are used for towelettes. Just before...

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ASTM E2197 – Quantitative Disk Carrier Test

The ASTM E2197 method is designed to test bactericidal, fungicidal, sporicidal, and virucidal capabilities of liquid germicides on non-porous hard surfaces using a 1 cm diameter brushed stainless steel surface. This method has been accepted by the U.S. EPA to substantiate efficacy claims for disinfectants against endospores of Clostridium difficile. The method...

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ASTM E2197 Disk-Carrier Test for C. difficile

ASTM E2197 Test: Procedure at a GlanceIn this study, efficacy of a test disinfectant is measured qualitatively against various vegetative bacteria, viruses, fungi, mycobacteria, or bacterial spores.  For EPA registration the disinfectant is tested at the lower certified limits (or minimum recommended concentration MRC), a dilution of the test...

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ASTM E2315 – Liquid Suspension Time-Kill Test

The liquid suspension time-kill test is excellent for disinfectant product developers because it is a fast, relatively inexpensive, and reproducible way to measure the biocidal potential of a liquid antimicrobial formulation. It consists of directly inoculating a liquid test substance with a high concentration of test microorganisms and then...

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AOAC Germicidal and Detergent Sanitizing Action of Disinfectants (AOAC 960.09)

The AOAC Germicidal and Detergent Sanitizing Action of Disinfectants Test Method is specified by US EPA as a method which can be used to substantiate efficacy claims for certain food-contact surface sanitizers. It is particularly appropriate for dilutable sanitizers that do not utilize halogens (chlorine, bromine). Test Method Summary Cultures...

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ASTM E2315 Time-Kill Test

ASTM E2315: Procedure at a GlanceThe Liquid Suspension Time-Kill begins with the direct inoculation of a volume of the disinfectant in question with a specified representative organism. After a specified contact time the system is neutralized and the presence of microorganisms is monitored and quantified following a period of...

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AOAC Germicidal Spray Products as Disinfectants Test

Procedure At A Glance For this method, sterile glass cover slips (carriers) are transferred into sterile petri plates then inoculated with 10 μl of the test bacteria. For products that only have a sanitizing/disinfecting claim, meaning a cleaning step to remove organic soil that precedes the sanitizing/disinfecting of the contaminated...

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Chlorine Equivalency Method for Sanitizers (AOAC 955.16)

Officially called the Chlorine (available) in Disinfectants Germicidal Equivalent Concentration, AOAC method 955.16 is typically used to test the efficacy of halogens such as chlorine, bromine, iodine. It is a rather unique method among disinfectant and sanitizer test methods, designed to identify the amount of a test product that is...

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AOAC Germicidal Spray Products Test (AOAC 961.02)

Originally developed in 1961, the AOAC Germicidal Spray Test is a standard method for evaluating the efficacy of liquid disinfectants on hard, non-porous surfaces. It is one of the EPA-specified test methods for antimicrobial pesticide registration. This method is particularly appropriate for disinfectants which do not require dilution and...

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Custom Disinfectant Compatibility Test

Custom Disinfectant Compatibility Test: Procedure at a GlanceIn addition to standard procedures for testing material compatibility, Microchem Laboratory can incorporate a variety of testing parameters into the determination of compability, such as:Exposure timeDrying timeMethod of dryingTemperatureHumidityMechanical abrasionUV exposureSimulated use between exposure cyclesAnd countless other options. In varying essential parameters...

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AOAC Germicidal Spray Test Modified for Fungi

Below, you will find a summary of the AOAC Germicidal Spray Products Test method, modified for use with fungi. The method is sometimes modified for use with fungi to support supplemental efficacy (fungicidal) label claims for pesticides registered with the United States Environmental Protection Agency. Trichophyton mentagtrophytes is the preferred test...

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EN 14476

Summary of the EN 14476 TestBefore test initiation the appropriate conditions for the test product are selected. Required conditions vary depending on the category of product and the conditions of use.The test substance is prepared according to Study Sponsor directions.A 0.8 ml aliquot of test substance is supplemented with...

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AOAC Use Dilution Test (AOAC 955.14; 955.15; 964.02)

The AOAC Use Dilution method is a method of testing the efficacy of disinfectants, originally developed in 1955. Throughout its numerous revisions, it has become the standard for evaluating liquid and dilutable liquid disinfectants for hard surfaces. In particular, this method is specified by the U.S. EPA as the...

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EN 1650 (Fungicidal Suspension Test for Disinfectants)

The EN 1650 test method, titled "Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic, and institutional areas", is a European Standard, used to determine the anti-fungal efficacy of chemical disinfectants and antiseptics used in...

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Virucidal Efficacy Tests for EPA

Virucides are regulated by the United States Environmental Protection Agency. Recent EPA guidelines provide companies with several different options for testing virucides, including ASTM E1053, and AOAC Germicidal Spray Products Test modified for viruses. Any type of virucide can be tested using ASTM E1053. If a company prefers to utilize...

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AOAC 955.17 Fungicidal Activity of Disinfectants

This test is used to assess the fungicidal activity of a disinfectant. In this test, a typical fungus strain of Trichophyton mentagrophytes is cultured and resuspended in order to attain spore producing mycelium; however, the method can be modified to meet the needs of the study sponsor's requirements. The...

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AOAC 966.04 Sporicidal Activity Test

AOAC 966.04 Sporicidal Activity Test: Procedure at a GlanceEssential for the registration and verification of sporicidal claims with the EPA as a countertop disinfectant and the FDA as a high-level disinfectant, the AOAC 966.04 Sporicidal Activity Test uses either Bacillus subtilis or Clostridium sporogenes as the model organism for...

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