EPA

EPA Residual Self-Disinfectant (RSD) Method

The residual self-disinfection (RSD) method follows EPA Protocol # 01-1A. This is the only method which can currently be used to substantiate long-term residual disinfectant claims with the EPA. It evaluates the residual disinfectant efficacy of antimicrobial products after application to inanimate, nonporous, and non-food contact hard surfaces. This test...

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Tuberculocidal Activity of Disinfectants (AOAC 965.12)

The AOAC Tuberculocidal Activity of Disinfectants method is specified by US EPA as a method which can be used to substantiate tuberculocidal efficacy claims for disinfectants. It is a modified version of the AOAC Use-Dilution test method and particularly appropriate for dilutable disinfectants. Summary of the AOAC Tuberculocidal Activity of...

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Virucidal Towelettes Effectiveness Test

Virucidal Efficacy Guidelines The U.S. EPA recommends the use of internationally-recognized standard test methods as the basis for pre-saturated towelette virucidal efficacy evaluations: AOAC Germicidal Spray Products as Disinfectants Test, Modified for Wipes Brief Summary of the Test The U.S. EPA recommends the use of internationally-recognized standard test methods as the basis for...

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EPA Test Method for the Residual Self-Sanitizing Activity of Copper Alloy Surfaces

The EPA's "Test Method for Residual Self-Sanitizing Activity of Copper Alloy Surfaces" is designed to quantitatively test the ability of copper alloy surfaces to kill 99.9% of the target microorganism for 24 hours, after multiple simulated wear cycles and re-inoculations. In order to obtain a residual self-sanitizer claim, an initial copper sufrace...

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Fungicidal Activity of Disinfectants (AOAC Method 955.17)

The AOAC Fungicidal Activity of Disinfectants Test method examines the fungicidal capabilities of water-soluble fungicides intended for use in disinfection of inanimate objects. The AOAC Fungicidal Activity of Disinfectants Test is specified by the US EPA as a method which can be used to substantiate fungicidal efficacy claims. Below, you...

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Pre-Saturated Towelette Disinfection Test

The EPA pre-saturated towelette disinfection test method is used to substantiate efficacy claims for disinfectant towelettes.     This method is a modification of the AOAC Germicidal Spray Products Test for Disinfectants. Instead of contaminated test surfaces being sprayed with disinfectant as in the germicidal spray products test, they are wiped with...

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EPA Test Method for the Continuous Reduction of Bacterial Contamination on Copper Alloy Surfaces

The EPA's "Test Method for the Continuous Reduction of Bacterial Contamination on Copper Alloy Surfaces" is designed to quantitatively test the ability of copper alloy surfaces to continuously reduce bacterial inoculums on these surfaces. It is a difficult test for most antimicrobial surfaces to pass. In order to obtain...

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ASTM E1053 – Surface Time Kill Test for Viruses

Summary of the ASTM E1053 Method ASTM E1053: Virucidal Efficacy of Hard Surface Disinfectants - Column Neutralization Preparation of Virus and Inoculation of Carriers: 100 x 15 mm Petri dishes are used for liquid and spray products, and 150 x 20 mm glass Petri dishes are used for towelettes. Just before...

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ASTM E1153 – EPA Non-Food Contact Sanitizer Test

The ASTM E1153 is a test method recognized by the EPA for the claim substantiation of sanitizers for use on non-food contact surfaces. For this reason, it is often referred to as the "non-food contact sanitizer" test. This test is performed by drying bacteria onto glass carriers, treating them with...

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ASTM E1153 Non-Food Contact Sanitizer Test

ASTM E1153: Procedure At a GlanceIn this study, carriers/surfaces will be inoculated with 10 µl of Staphylococcus aureus and Klebsiella pneumoniae (or Enterobacter aerogenes substituted) and dried for 35 minutes at 35 ± 2ºC.  The surfaces will be transferred into sterile jars, petri plates, or other appropriate sterile container...

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AOAC Germicidal and Detergent Sanitizing Action of Disinfectants (AOAC 960.09)

The AOAC Germicidal and Detergent Sanitizing Action of Disinfectants Test Method is specified by US EPA as a method which can be used to substantiate efficacy claims for certain food-contact surface sanitizers. It is particularly appropriate for dilutable sanitizers that do not utilize halogens (chlorine, bromine). Test Method Summary Cultures...

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ASTM E2197 – Quantitative Disk Carrier Test

The ASTM E2197 method is designed to test bactericidal, fungicidal, sporicidal, and virucidal capabilities of liquid germicides on non-porous hard surfaces using a 1 cm diameter brushed stainless steel surface. This method has been accepted by the U.S. EPA to substantiate efficacy claims for disinfectants against endospores of Clostridium difficile. The method...

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AOAC Germicidal Spray Products as Disinfectants Test

Procedure At A Glance For this method, sterile glass cover slips (carriers) are transferred into sterile petri plates then inoculated with 10 μl of the test bacteria. For products that only have a sanitizing/disinfecting claim, meaning a cleaning step to remove organic soil that precedes the sanitizing/disinfecting of the contaminated...

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ASTM E2197 Disk-Carrier Test for C. difficile

ASTM E2197 Test: Procedure at a GlanceIn this study, efficacy of a test disinfectant is measured qualitatively against various vegetative bacteria, viruses, fungi, mycobacteria, or bacterial spores.  For EPA registration the disinfectant is tested at the lower certified limits (or minimum recommended concentration MRC), a dilution of the test...

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AOAC Germicidal Spray Products Test (AOAC 961.02)

Originally developed in 1961, the AOAC Germicidal Spray Test is a standard method for evaluating the efficacy of liquid disinfectants on hard, non-porous surfaces. It is one of the EPA-specified test methods for antimicrobial pesticide registration. This method is particularly appropriate for disinfectants which do not require dilution and...

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Chlorine Equivalency Method for Sanitizers (AOAC 955.16)

Officially called the Chlorine (available) in Disinfectants Germicidal Equivalent Concentration, AOAC method 955.16 is typically used to test the efficacy of halogens such as chlorine, bromine, iodine. It is a rather unique method among disinfectant and sanitizer test methods, designed to identify the amount of a test product that is...

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AOAC Germicidal Spray Test Modified for Fungi

Below, you will find a summary of the AOAC Germicidal Spray Products Test method, modified for use with fungi. The method is sometimes modified for use with fungi to support supplemental efficacy (fungicidal) label claims for pesticides registered with the United States Environmental Protection Agency. Trichophyton mentagtrophytes is the preferred test...

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Custom Virucidal Efficacy Testing

Custom Study Set-Up Custom virucidal studies can be performed for device testing, on-site antimicrobial active ingredient generators, and many other product types. All studies can be conducted according to EPA and FDA regulatory efficacy requirements, and customized according to the unique needs and timelines of the Study Sponsor with the...

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AOAC Germicidal Spray Test, Modified for Viruses

Originally developed in 1961, the AOAC Germicidal Spray Test is a standard method for evaluating the efficacy of liquid disinfectants on hard non-porous surfaces. This method is particularly appropriate for disinfectants which do not require dilution and are designed to be used with an aerosol or trigger spray. This...

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EPA Copper Surfaces as Sanitizers Test

The EPA's "Test method for Efficacy of Copper Alloy Surfaces as a Sanitizer" is designed to quantitatively test the ability of copper and copper alloy surfaces to act as a sanitizer. Most importantly, this method serves as the technical basis for substantiation of so-called "health claims" for antimicrobial surfaces. In other...

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AOAC Use Dilution Test (AOAC 955.14; 955.15; 964.02)

The AOAC Use Dilution method is a method of testing the efficacy of disinfectants, originally developed in 1955. Throughout its numerous revisions, it has become the standard for evaluating liquid and dilutable liquid disinfectants for hard surfaces. In particular, this method is specified by the U.S. EPA as the...

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EPA Disinfectant Towelette Test

EPA Towelette Test: Procedure at a glanceThe typical bacteria used in this test, S. aureus, P. aeruginosa, and S. enterica are representative Gram-positive and Gram-negative pathogenic bacteria. The microorganisms are inoculated and dried onto the surface of glass microscope slides, the carrier. The slides are then wiped with pre-saturated disinfectant...

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AOAC Use-Dilution Test

AOAC Use Dilution Test: Procedure at a GlanceFor this study, bactericidal activity is measured qualitatively against various microorganisms.  Sterile test surfaces called penicylinders are inoculated with culture, dried, and then exposed to the test disinfectant at the label claim contact temperature and time.  The carriers are then transferred to...

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EPA Test Method for Residual Self-Sanitizing Activity of Dried Chemical Residues on Hard, Non-Porous Surfaces

The residual self-sanitization (RSS) method, EPA Protocol # 01-1A, is the only method which can currently be used to substantiate long-term sanitization claims with the EPA. It evaluates the residual sanitizing efficacy of antimicrobial products after application to inanimate, nonporous, and non-food contact hard surfaces. This test method was initially...

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ASTM E1052 – Suspension Time-Kill Test for Virus

Summary of the ASTM E1052 Test ASTM E1052: Standard Practice to Assess the Activity of Microbicides Against Virucides in Suspension The frozen stock test virus is thawed and diluted to a titer of approximately 6-log10 infectious units per 0.1 ml. If requested by the Study Sponsor, the test virus is...

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Virucidal Efficacy Tests for EPA

Virucides are regulated by the United States Environmental Protection Agency. Recent EPA guidelines provide companies with several different options for testing virucides, including ASTM E1053, and AOAC Germicidal Spray Products Test modified for viruses. Any type of virucide can be tested using ASTM E1053. If a company prefers to utilize...

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AOAC 966.04 Sporicidal Activity Test

AOAC 966.04 Sporicidal Activity Test: Procedure at a GlanceEssential for the registration and verification of sporicidal claims with the EPA as a countertop disinfectant and the FDA as a high-level disinfectant, the AOAC 966.04 Sporicidal Activity Test uses either Bacillus subtilis or Clostridium sporogenes as the model organism for...

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