Hard Surface

Hard Surface Time-Kill Test for Disinfectants

Summary of the Hard Surface Time-Kill Test     A microbial culture is prepared. For most bacteria, a 24 hour culture in nutrient broth works well. For most fungi, a spore preparation from a saline wash works well.   A volume of microbial culture (usually 0.010 ml to 0.020 ml) is placed onto the...

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ASTM E1053 – Surface Time Kill Test for Viruses

Summary of the ASTM E1053 Method ASTM E1053: Virucidal Efficacy of Hard Surface Disinfectants - Column Neutralization Preparation of Virus and Inoculation of Carriers: 100 x 15 mm Petri dishes are used for liquid and spray products, and 150 x 20 mm glass Petri dishes are used for towelettes. Just before...

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EPA Test Method for Residual Self-Sanitizing Activity of Dried Chemical Residues on Hard, Non-Porous Surfaces

The residual self-sanitization (RSS) method, EPA Protocol # 01-1A, is the only method which can currently be used to substantiate long-term sanitization claims with the EPA. It evaluates the residual sanitizing efficacy of antimicrobial products after application to inanimate, nonporous, and non-food contact hard surfaces. This test method was initially...

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