- Standardize inoculum diluent. Results from the AATCC 100 test method are greatly influenced by the medium used to dilute the culture prior to inoculation of fabrics. Most people in the industry use saline. This produces the most microbial reduction, but limits assessment of growth-inhibiting effects.
- Standardize swatches. ATL finds that when the inoculum volume is held to 1.0 ml, physical standardization of swatches enhances reproducibility. We recommend circular 5 cm diameter swatches, stacked approximately 2-4 high.
- Fully neutralize antimicrobial agents. Insufficient neutralization of antimicrobials is a heart-breaker – it can make a person think a fabric is spectacularly bactericidal when in fact it may only be bacteriostatic.
- Make sure the inoculum does not pool or puddle beneath the inoculated fabric. Liquid microenvironments physically separated from the antimicrobial agent reduce overall microbial reductions.
4 Tips for AATCC 100 Fabric TestingBen Tanner