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Structure and Physiology
This bacteria is a Gram-positive, rod shaped, endospore generating obligate anaerobe. Clostridium species are part of the normal human gut flora that produce spores which are highly resistant to chemical and environmental conditions.
Transmission and Disease
C. difficile is commonly associated with hospital acquired infections and is known to cause antibiotic assisted colitis.
Because of its high resistance to antimicrobials, C. difficile is a benchmark bacteria for sporicidal and sterilant activity of chemicals.
C. difficile requires a slightly longer incubation period of 48 to 72 hours in an anaerobic chamber.